A Gain-of-Function Mutation in Gating of Corynebacterium glutamicum NCgl1221 Causes Constitutive Glutamate Secretion

• DOI: 10.1128/aem.01310-12
• Publication Date: 2012-05-20T12:54:07Z
• Authors: Yoshitaka Nakayama; Kenjiro Yoshimura; Hidetoshi Iida
• Keywords: Corynebacterium glutamicum; 0303 health sciences; 03 medical and health sciences; Patch-Clamp Techniques; Amino Acid Transport Systems; Bacterial Proteins; Species Specificity; Molecular Sequence Data; Mutation, Missense; Glutamic Acid; Amino Acid Sequence; Ion Channel Gating

ABSTRACT

The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum . Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.