Comparison of Cyanopeptolin Genes in Planktothrix , Microcystis , and Anabaena Strains: Evidence for Independent Evolution within Each Genus

• DOI: 10.1128/aem.01475-07
• Publication Date: 2007-10-06T00:35:06Z
• Authors: Trine B. Rounge; Thomas Rohrlack; Ave Tooming-Klund...; Tom Kristensen; Kjetill S. Jakobsen
• Keywords: Nonribosomal peptide; Horizontal Gene Transfer; Gene cluster; Microcystis

The major cyclic peptide cyanopeptolin 1138, produced by Planktothrix strain NIVA CYA 116, was characterized and shown to be structurally very close the earlier-characterized oscillapeptin E. A gene cluster likely encode corresponding synthetase sequenced from same strain. 30-kb oci contains two novel domains previously not detected in nonribosomal clusters (a putative glyceric acid-activating domain a sulfotransferase domain), addition seven modules. Unlike described Anabaena Microcystis, halogenase is present. three show similar arrangements, while binding pocket signatures deduced adenylation sequences additional tailoring vary. This suggests loss gain of within each genus, after diversification clades, as events leading present diversity. ABC transporter genes associated with form monophyletic clade accordingly are have evolved part functional unit. Phylogenetic analyses condensation domains, including cyanopeptolins microcystins, closer similarity between Microcystis than these domain. No clear evidence recombination microcystins could detected. There were no strong indications horizontal transfer across genera, supporting independent evolution genus.