In Vivo and Ex Vivo Evaluations of Bacteriophages e11/2 and e4/1c for Use in the Control of Escherichia coli O157:H7

• DOI: 10.1128/aem.01530-10
• Publication Date: 2010-09-18T01:12:08Z
• Authors: Lucia Rivas; Brid Coffey; Olivia McAuliffe; Mary J. McDonnell; Catherine M. Burgess; Aidan Coffey; R. Paul Ross; Geraldine Duffy
• Keywords: Ex vivo

This study investigated the effect of bacteriophages (phages) e11/2 and e4/1c against Escherichia coli O157:H7 in an ex vivo rumen model cattle vivo. In model, samples were inoculated with either 10³ or 10⁶ CFU/ml inoculum E. challenged separately each bacteriophage. presence phage e11/2, numbers bacteria significantly (P < 0.05) reduced to below limit detection within 1 h. Phage 2 h incubation, but number surviving then remained unchanged over a further 22-h incubation period. The ability cocktail reduce fecal shedding experimentally was two trials. Cattle (yearlings, n = 20 for trial one; adult fistulated cattle, two) orally 10(10) CFU O157:H7. Animals (n 10 dosed daily bacteriophage 10(11) PFU 3 days postinoculation. and/or determined 7 rapidly declined all animals 24 48 h; however, there no significant difference > between shed by phage-treated control animals. Phages recovered from not feces animal yearling one. While results suggest that phages are effective rumen, research is required improve antimicrobial effectiveness elimination