The Cyclic AMP Receptor Protein Modulates Colonial Morphology in Vibrio cholerae

• DOI: 10.1128/aem.01564-07
• Publication Date: 2007-10-06T00:35:06Z
• Authors: Weili Liang; Anisia J. Silva; Jorge A. Benitez
• Keywords: cAMP receptor protein; Wild type; Ectopic expression

Inactivation of the quorum-sensing regulator HapR causes Vibrio cholerae El Tor biotype strain C7258 to adopt a rugose colonial morphology that correlates with enhanced biofilm formation. V. mutants lacking cyclic AMP (cAMP) receptor protein (CRP) produce very little HapR, which results in elevated expression exopolysaccharide (vps) genes and compared wild type. However, Deltacrp still exhibited smooth expressed reduced levels vps isogenic hapR mutants. In this study we demonstrate deletion crp cya (adenylate cyclase) converts DeltahapR mutant one. The Deltacya double could be converted back by complementation cya, respectively. CRP was found enhance VpsR, strong activator expression, but diminish transcription VpsT. Ectopic VpsR restored morphology. Lowering intracellular cAMP addition glucose diminished rugosity. On basis our results, propose model for regulatory input on biosynthesis.