Two Novel Toxin Variants Revealed by Whole-Genome Sequencing of 175 Clostridium botulinum Type E Strains

• DOI: 10.1128/aem.01573-14
• Publication Date: 2014-08-09T04:55:20Z
• Authors: K. A. Weedmark; D. L. Lambert; P. Mabon; K. L. Hayden; C. J. Urfano; D. Leclair; G. Van Domselaar; J. W. Austin; C. R. Corbett
• Keywords: Clostridium botulinum; Neurotoxin; Botulism

We sequenced 175 Clostridium botulinum type E strains isolated from food, clinical, and environmental sources northern Canada analyzed their neurotoxin (bont) coding sequences (CDSs). In addition to bont/E1 bont/E3 variant types, sequence analysis identified two novel BoNT variants termed E10 E11. Strains producing were found along the eastern coastlines of Hudson Bay shores Ungava Bay, while E11 only in Koksoak River region Nunavik. BoNT/E3 widespread throughout Canada, with exception coast Bay.