Improved Production of Homo- d -Lactic Acid via Xylose Fermentation by Introduction of Xylose Assimilation Genes and Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in l -Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum

• DOI: 10.1128/aem.01692-09
• Publication Date: 2009-10-10T01:44:59Z
• Authors: Kenji Okano; Shogo Yoshida; Ryosuke Yamada; Tsutomu Tanaka; Chiaki Ogino; Hideki Fukuda; Akihiko Kondo
• Keywords: Transketolase; Pentose; Mixed acid fermentation

ABSTRACT The production of optically pure d -lactic acid via xylose fermentation was achieved by using a Lactobacillus plantarum NCIMB 8826 strain whose l -lactate dehydrogenase gene deficient and phosphoketolase genes were replaced with heterologous transketolase gene. After 60 h fermentation, 41.2 g/liter produced from 50 xylose.