Comparison of the Levels of Bacterial Diversity in Freshwater, Intertidal Wetland, and Marine Sediments by Using Millions of Illumina Tags

• DOI: 10.1128/aem.01821-12
• Publication Date: 2012-09-23T00:26:42Z
• Authors: Yu Wang; Hua-Fang Sheng; Yan He; Jin-Ya Wu; Yun-Xia Jiang; Nora Fung-Yee Tam; Hong-Wei Zhou
• Keywords: Deltaproteobacteria; Betaproteobacteria; Acidobacteria; Verrucomicrobia; Alphaproteobacteria; Planctomycetes

Sediment, a special realm in aquatic environments, has high microbial diversity. While there are numerous reports about the community marine sediment, freshwater and intertidal sediment communities have been overlooked. The present study determined millions of Illumina reads for comparison bacterial freshwater, wetland, sediments along Pearl River, China, using technically consistent approach. Our results show that both taxon richness evenness were highest medium lowest sediment. number sequences allowed determination wide variety lineages all reliable statistical analyses. Principal component analysis showed three types could be well separated from phylum to operational taxonomy unit (OTU) levels, OTUs abundant rare satisfactory resolutions. Statistical (LEfSe) demonstrated was enriched with Acidobacteria, Nitrospira, Verrucomicrobia, Alphaproteobacteria, Betaproteobacteria. had unique diverse primary producers (such as Chloroflexi, Bacillariophyta, Gammaproteobacteria, Epsilonproteobacteria) saprophytic microbes Actinomycetales, Bacteroidetes, Firmicutes). higher abundance Gammaproteobacteria Deltaproteobacteria, which mainly involved sulfate reduction anaerobic conditions. These helpful systematic understanding natural environments.