Evaluation of Two Surface Sampling Methods for Detection of Erwinia herbicola on a Variety of Materials by Culture and Quantitative PCR

• DOI: 10.1128/aem.01825-06
• Publication Date: 2007-04-07T00:24:07Z
• Authors: Mark P. Buttner; Patricia Cruz; Linda D. Stetzenbach; Tracy Cronin
• Keywords: Enumeration; Aerosolization

ABSTRACT This research was designed to evaluate surface sampling protocols for use with culture and quantitative PCR (QPCR) amplification assay detection of the gram-negative bacterial biothreat simulant Erwinia herbicola on a variety materials. Surfaces selected evaluation were wood laminate, glass computer monitor screens, metal file cabinets, plastic arena seats, nylon seat cushions, finished concrete flooring, vinyl tile flooring. Laboratory test chamber studies performed two methods, sponge macrofoam swab, E. In laboratory trials, seven materials inoculated known concentration cells samples collected from surfaces determine efficiencies. Culture analysis ineffective assessing collection efficiency because very few culturable obtained samples. QPCR demonstrated that DNA present in high concentrations all samples, efficiencies ranged 0.7 52.2%, depending method material. The swab generally more efficient than surfaces. Test trials also which aerosolized into allowed settle onto Surface results supported those trials. this study demonstrate capabilities enhance enumeration biocontaminants provide information comparability methods.