Yeast Populations Residing on Healthy orBotrytis-Infected Grapes from a Vineyard in Attica, Greece

0301 basic medicine 0303 health sciences Greece Molecular Sequence Data Biodiversity Sequence Analysis, DNA DNA, Ribosomal 03 medical and health sciences Sequence Homology, Nucleic Acid Yeasts DNA, Ribosomal Spacer Vitis Botrytis DNA, Fungal Deoxyribonucleases, Type II Site-Specific Phylogeny Polymorphism, Restriction Fragment Length Plant Diseases
DOI: 10.1128/aem.01864-06 Publication Date: 2007-02-10T02:15:25Z
ABSTRACT
ABSTRACTThe yeast flora associated with healthy andBotrytis-infected grapes was assessed. Molecular identification methods assigned isolates to six genera and nine species. For the first timeHanseniaspora opuntiaewas encountered as an inhabitant of the grape ecosystem. By using DraI, an informative restriction fragment length polymorphism pattern was generated to distinguishH. opuntiaefrom the closely related organismHanseniaspora guilliermondii. Botrytisinfection resulted in a larger population and greater diversity of yeasts enriched with fermentative or spoilage species.
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