Bacterial Polyester Inclusions Engineered To Display Vaccine Candidate Antigens for Use as a Novel Class of Safe and Efficient Vaccine Delivery Agents

• DOI: 10.1128/aem.01965-09
• Publication Date: 2009-10-17T00:59:01Z
• Authors: Natalie A. Parlane; D. Neil Wedlock; Bryce M. Buddle; Bernd H. A. Rehm
• Keywords: ESAT-6; Humoral immunity

Bioengineered bacterial polyester inclusions have the potential to be used as a vaccine delivery system. The biopolyester beads were engineered display fusion protein of synthase PhaC and two key antigens involved in immune response infectious agent that causes tuberculosis, Mycobacterium notably antigen 85A (Ag85A) 6-kDa early secreted antigenic target (ESAT-6) from tuberculosis. Polyester displaying respective at high density successfully produced (henceforth called Ag85A-ESAT-6 beads) by recombinant Escherichia coli. ability enhance mouse immunity displayed was investigated. not toxic animals, determined weight gain absence lesions inoculation site immunized animals. In vivo injection mice induced significant humoral cell-mediated responses both Ag85A ESAT-6. Vaccination with efficient stimulating on their own, this enhanced administration an oil-in-water emulsion. addition, vaccination significantly stronger than equivalent dose alone. mixed Th1/Th2 nature, assessed induction cytokine gamma interferon (Th1 response) increased levels immunoglobulin G1 (Th2 response). Hence, foreign represent new class versatile, safe, biocompatible vaccines.