Actively Growing Bacteria in the Inland Sea of Japan, Identified by Combined Bromodeoxyuridine Immunocapture and Denaturing Gradient Gel Electrophoresis

• DOI: 10.1128/aem.02111-06
• Publication Date: 2007-03-03T02:13:21Z
• Authors: Koji Hamasaki; Akito Taniguchi; Yuya Tada; Richard A. Long; Farooq Azam
• Keywords: Bromodeoxyuridine

ABSTRACT A fundamental question in microbial oceanography concerns the relationship between prokaryote diversity and biogeochemical function an ecosystem context. We combined bromodeoxyuridine (BrdU) magnetic bead immunocapture PCR-denaturing gradient gel electrophoresis (BUMP-DGGE) to examine phylotype-specific growth natural marine assemblages. also examined a broad range of bacterial isolates determine their abilities incorporate BrdU order test validity method for application diverse found that 27 29 belonging different taxa could BrdU. BUMP-DGGE analysis revealed phylogenetic affiliations DNA-synthesizing, presumably actively growing bacteria across eutrophic mesotrophic transect Inland Sea Japan. BrdU-incorporating (growing) communities were substantially from total communities. The majority (34/56) phylotypes incorporated growing, these comprised 10 alphaproteobacteria, 1 betaproteobacterium, 11 gammaproteobacteria, Cytophaga-Flavobacterium-Bacteroides group bacteria, unclassified bacterium. All BrdU-responsive alphaproteobacteria members Rhodobacterales , suggesting such dominant alphaproteobacterial populations our samples. gammaproteobacteria belonged Oceanospirillales SAR86 cluster, Pseudomonadales Alteromonadales Vibrionales . Thus, contemporaneous cooccurrence was consistent pattern biogeochemically varied study area.