Inactivation of a Norovirus by High-Pressure Processing

• DOI: 10.1128/aem.02117-06
• Publication Date: 2006-12-02T01:34:46Z
• Authors: David H. Kingsley; Daniel R. Holliman; Kevin R. Calci; Haiqiang Chen; George J. Flick
• Keywords: Murine norovirus; Pascalization; Virus inactivation; Log reduction; Strain (injury)

Murine norovirus (strain MNV-1), a propagable norovirus, was evaluated for susceptibility to high-pressure processing. Experiments with virus stocks in Dulbecco's modified Eagle medium demonstrated that at room temperature (20 degrees C) the inactivated over pressure range of 350 450 MPa, 5-min, 450-MPa treatment being sufficient inactivate 6.85 log(10) PFU MNV-1. The inactivation MNV-1 enhanced when applied an initial 5 C; 5-min MPa 30 C 1.15 virus, while same resulted reduction 5.56 PFU. Evaluation as function times ranging from 0 150 s and 900 20 C, respectively, indicated decreasing rate time consistent Weibull or log-logistic kinetics. directly within oyster tissues demonstrated; 400-MPa 4.05 This work is first demonstration can be by high suggests good prospects nonpropagable human strains foods.