Inactivation Mechanism and Efficacy of Grape Seed Extract for Human Norovirus Surrogate

• DOI: 10.1128/aem.02247-21
• Publication Date: 2022-04-25T08:30:20Z
• Authors: Chamteut Oh; Ratul Chowdhury; Laxmicharan Samineni; Joanna L. Shisler; Manish Kumar; Thanh H. Nguyen
• Keywords: Acute gastroenteritis; Waterborne diseases; Human pathogen; Contaminated food

Proper disinfection of harvested food and water is critical to minimize infectious disease. Grape seed extract (GSE), a commonly used health supplement, mixture plant-derived polyphenols. Polyphenols possess antimicrobial antifungal properties, but antiviral effects are not well-known. Here we show that GSE outperformed chemical disinfectants (e.g., free chlorine peracetic acids) in inactivating Tulane virus, human norovirus surrogate. induced virus aggregation, process correlated with decrease titers. This aggregation were reversible. Molecular docking simulations indicate polyphenols potentially formed hydrogen bonds strong hydrophobic interactions specific residues viral capsid proteins. Together, these data suggest physically associate proteins aggregate viruses as means inhibit entry into the host cell. Plant-based like an attractive alternative remove from or food. IMPORTANCE Human noroviruses major food- waterborne pathogens, causing approximately 20% all cases acute gastroenteritis developing developed countries. sanitation strategies norovirus-caused disease until reliable vaccine created. (GSE) supplement. known for antimicrobial, antifungal, antibiofilm activities, In studies presented here, (i.e., Based on molecular assays simulations, current model bind capsid, event triggers virion aggregation. It thought this prevents entering cells.