Exploiting the Natural Diversity of RhlA Acyltransferases for the Synthesis of the Rhamnolipid Precursor 3-(3-Hydroxyalkanoyloxy)Alkanoic Acid

• DOI: 10.1128/aem.02317-19
• Publication Date: 2020-01-13T12:43:47Z
• Authors: Andrea Germer; Till Tiso; Conrad Müller; Beate Behrens; Christian Vosse; Karen Scholz; Matti Froning; Heiko Hayen; Lars M. Blank
• Keywords: info:eu-repo/classification/ddc/570; 0106 biological sciences; 0301 basic medicine; 570; Bacteria; Carboxylic Acids; 01 natural sciences; 03 medical and health sciences; Bacterial Proteins; Glycolipids; Acyltransferases; Biotechnology

The RhlA specificity explains the observed differences in 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) congeners. Whole-cell catalysts can now be designed for the synthesis of different congener mixtures of HAAs and rhamnolipids, thereby contributing to the envisaged synthesis of designer HAAs.