Use of Flow Cytometry for Rapid, Quantitative Detection of Poliovirus-Infected Cells via TAT Peptide-Delivered Molecular Beacons

• DOI: 10.1128/aem.02429-12
• Publication Date: 2012-11-17T05:52:23Z
• Authors: Divya Sivaraman; Hsiao-Yun Yeh; Ashok Mulchandani; Marylynn V. Yates; Wilfred Chen
• Keywords: Molecular beacon

Rapid and efficient detection of viral infection is crucial for the prevention disease spread during an outbreak timely clinical management. In this paper, utility Tat peptide-modified molecular beacons (MBs) as a rapid diagnostic tool virus-infected cells was demonstrated. The intracellular delivery mediated by peptide enabled infected within 30 s, reaching saturation in signal min. This scheme coupled with flow cytometry (FC), resulting automated, high-throughput method identification cells. Because 2-order-of-magnitude difference fluorescence intensity between uninfected cells, few 1% could be detected. its speed sensitivity, approach may adapted practical diagnosis multiple infections.