Hollow-Fiber Membrane Chamber as a Device for In Situ Environmental Cultivation

• DOI: 10.1128/aem.02542-08
• Publication Date: 2009-03-30T16:25:29Z
• Authors: Yoshiteru Aoi; Tomoyuki Kinoshita; Toru Hata; Hiroaki Ohta; Haruko Obokata; Satoshi Tsuneda
• Keywords: Petri dish

ABSTRACT A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces porous connected with injectors. allows rapid exchange chemical compounds, thereby simulating a natural environment. Comparative analysis through the three types samples performed using this newly designed and conventional agar-based petri dish. results show that ratios novel phylotypes isolates, species-level diversities, cultivabilities HFMC-based are higher than those dish all samples, suggesting new is effective various