Microbial Diversity in the Early In Vivo -Formed Dental Biofilm

• DOI: 10.1128/aem.03984-15
• Publication Date: 2016-01-09T03:59:34Z
• Authors: D. Heller; E. J. Helmerhorst; A. C. Gower; W. L. Siqueira; B. J. Paster; F. G. Oppenheim
• Keywords: Dental plaque

Although the mature dental biofilm composition is well studied, there very little information on earliest phase of in vivo tooth colonization. Progress collection methodologies and techniques large-scale microbial identification have made new studies this field oral biology feasible. The aim study was to characterize temporal changes diversity cultivable noncultivable microbes early biofilm. Samples were collected from 11 healthy subjects at 0, 2, 4, 6 h after removal plaque pellicle surfaces. With semiquantitative Human Oral Microbiome Identification Microarray (HOMIM) technique, which based 16S rRNA sequence hybridizations, samples analyzed with currently available 407 HOMIM probes. This led least 92 species, streptococci being most abundant bacteria across all time points subjects. High-frequency detection also Haemophilus parainfluenzae, Gemella haemolysans, Slackia exigua, Rothia species. Abundance over noted for Streptococcus anginosus intermedius (P = 0.02), mitis bv. 2 0.0002), oralis cluster I 0.003), G. haemolysans 0.0005), Stenotrophomonas maltophilia 0.02). Among uncultivable microbiota, eight phylotypes detected stages formation, one belonging candidate bacterial division TM7, has attracted attention due its potential association periodontal disease.