Evaluation of a Disinfectant Wipe Intervention on Fomite-to-Finger Microbial Transfer

• DOI: 10.1128/aem.04235-13
• Publication Date: 2014-03-08T05:40:12Z
• Authors: Gerardo U. Lopez; Masaaki Kitajima; Aaron Havas; Charles P. Gerba; Kelly A. Reynolds
• Keywords: Disinfection; Fingers; 0301 basic medicine; 03 medical and health sciences; Bacteria; Fomites; Viruses; Humans; 6. Clean water; Disinfectants

ABSTRACT

Inanimate surfaces, or fomites, can serve as routes of transmission of enteric and respiratory pathogens. No previous studies have evaluated the impact of surface disinfection on the level of pathogen transfer from fomites to fingers. Thus, the present study investigated the change in microbial transfer from contaminated fomites to fingers following disinfecting wipe use. Escherichia coli (10 8 to 10 9 CFU/ml), Staphylococcus aureus (10 9 CFU/ml), Bacillus thuringiensis spores (10 7 to 10 8 CFU/ml), and poliovirus 1 (10 8 PFU/ml) were seeded on ceramic tile, laminate, and granite in 10-μl drops and allowed to dry for 30 min at a relative humidity of 15 to 32%. The seeded fomites were treated with a disinfectant wipe and allowed to dry for an additional 10 min. Fomite-to-finger transfer trials were conducted to measure concentrations of transferred microorganisms on the fingers after the disinfectant wipe intervention. The mean log 10 reduction of the test microorganisms on fomites by the disinfectant wipe treatment varied from 1.9 to 5.0, depending on the microorganism and the fomite. Microbial transfer from disinfectant-wipe-treated fomites was lower (up to <0.1% on average) than from nontreated surfaces (up to 36.3% on average, reported in our previous study) for all types of microorganisms and fomites. This is the first study quantifying microbial transfer from contaminated fomites to fingers after the use of disinfectant wipe intervention. The data generated in the present study can be used in quantitative microbial risk assessment models to predict the effect of disinfectant wipes in reducing microbial exposure.