Inactivation of Human Norovirus in Contaminated Oysters and Clams by High Hydrostatic Pressure

• DOI: 10.1128/aem.04260-13
• Publication Date: 2014-02-01T05:27:25Z
• Authors: Mu Ye; Xinhui Li; David H. Kingsley; Xi Jiang; Haiqiang Chen
• Keywords: Hydrostatic pressure; Infectivity; Ostreidae

ABSTRACT Human norovirus (NoV) is the most frequent causative agent of food-borne disease associated with shellfish consumption. In this study, effect high hydrostatic pressure (HHP) on inactivation NoV was determined. Genogroup I.1 (GI.1) or genogroup II.4 (GII.4) inoculated into oyster homogenates and treated at 300 to 600 MPa 25, 6, 1°C for 5 min. After HHP, samples were RNase viral particles extracted porcine gastric mucin (PGM)-conjugated magnetic beads (PGM-MBs). Viral RNA then quantified by real-time reverse transcription (RT)-PCR. Since PGM contains histo-blood group-like antigens, which can act as receptors NoV, deficiency binding an indication loss infectivity NoV. PGM-MBs, RT-PCR-detectable in oysters reduced 0.4 >4 log 10 HHP MPa. The GI.1 more resistant than GII.4 ( P < 0.05). lower temperatures significantly enhanced Pressure treatment also conducted clam homogenates. Treatment 450 achieved a reduction both It therefore concluded that could be applied potential intervention inactivating raw shellfish. method pretreatment RNase, extraction using PGM-MB binding, quantification RT-PCR explored practical means distinguishing between infectious noninfectious