Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment
Microcosm
Viable but nonculturable
Viable count
Campylobacteriosis
Acridine orange
DOI:
10.1128/aem.52.3.531-538.1986
Publication Date:
2020-01-01T03:56:21Z
AUTHORS (2)
ABSTRACT
Conditions influencing the survival of Campylobacter jejuni in natural aquatic environment have been determined. Release spp. into waters by animal hosts is postulated to play a key role maintenance viability and transmission organism environment. Laboratory flask microcosms containing filter-sterilized stream water were used test C. for ability remain viable simulated systems. The compared with biphasic shaking broth procedures routinely growth research laboratory. stream-water analyzed determine effects temperature aeration on well-characterized strain isolated originally from human campylobacteriosis patient. Morphological characteristics evaluated phase-contrast microscopy scanning or electron microscopy. Survival curves quantified basis plate counts, epifluorescent microscopy, optical density measurements, direct counts associated protein synthesis absence DNA replication. A significant difference was observed between results enumeration, i.e., acridine orange those spread cultures. In all cases, increasing cultivation resulted decreased recoverability laboratory media, due possibly an increased metabolic rate, as CO2 evolution presence radiolabeled glutamate. Stream held at low (4 degrees C) sustained numbers campylobacters greater than 4 months. Microcosms, aerated shaking, exhibited logarithmic decline recoverable jejuni, while stationary systems underwent more moderate rate decrease nonculturable state.(ABSTRACT TRUNCATED AT 250 WORDS)
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