Direct measurements of natural planktonic bacterial community viability by flow cytometry

0301 basic medicine 570 0303 health sciences 03 medical and health sciences 6. Clean water
DOI: 10.1128/aem.61.7.2783-2786.1995 Publication Date: 2020-01-06T18:09:33Z
ABSTRACT
A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B with a detergent-mediated permeabilization step. The viable bacterial count under optimal conditions was 7% in oligotrophic lake water and 75% in polluted river water.
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