Direct measurements of natural planktonic bacterial community viability by flow cytometry

• DOI: 10.1128/aem.61.7.2783-2786.1995
• Publication Date: 2020-01-06T18:09:33Z
• Authors: J Porter; J Diaper; C Edwards; R Pickup
• Keywords: Fluorescent labelling; Cytometry

A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B a detergent-mediated permeabilization step. The bacterial count under optimal conditions 7% oligotrophic lake water and 75% polluted river water.