Direct measurements of natural planktonic bacterial community viability by flow cytometry
Fluorescent labelling
Cytometry
DOI:
10.1128/aem.61.7.2783-2786.1995
Publication Date:
2020-01-06T18:09:33Z
AUTHORS (4)
ABSTRACT
A range of fluorescent viability dyes were used in conjunction with flow cytometry to rapidly enumerate viable bacteria from freshwater environments. Optimal labelling was achieved by using carboxyfluorescein diacetate or chemchrome B a detergent-mediated permeabilization step. The bacterial count under optimal conditions 7% oligotrophic lake water and 75% polluted river water.
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