Activity and three-dimensional distribution of toluene-degrading Pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridization and scanning confocal laser microscopy

• DOI: 10.1128/aem.62.12.4632-4640.1996
• Publication Date: 2020-01-06T18:14:48Z
• Authors: S Møller; A R Pedersen; L K Poulsen; E Arvin; S Molin
• Keywords: Pseudomonas putida; Acridine orange; Pseudomonadales

As a representative member of the toluene-degrading population in biofilter for waste gas treatment, Pseudomonas putida was investigated with 16S rRNA targeting probe. The three-dimensional distribution P. visualized biofilm matrix by scanning confocal laser microscopy, demonstrating that present throughout biofilm. Acridine orange staining revealed very heterogeneous structure fully hydrated biofilm, cell-free channels extending from surface into This indicated toluene may penetrate to deeper layers and consequently be actively degrading all regions Furthermore, measurements growth rate-related parameters showed reduced content cell size (relative batch culture), indicating not at maximal rate environment. Assuming reflected cellular activity, lower degradation could estimated. calculation responsible significant part (65%) degraded entire community.