Dynamics of a microbial community associated with manure hot spots as revealed by phospholipid fatty acid analyses

• DOI: 10.1128/aem.63.6.2224-2231.1997
• Publication Date: 2020-01-06T18:28:49Z
• Authors: A Frostegård; S O Petersen; E Bååth; T H Nielsen
• Keywords: 2. Zero hunger; Time Factors; Fatty Acids; 04 agricultural and veterinary sciences; Biological Sciences; 15. Life on land; Feces; Soil; Animals; 0401 agriculture, forestry, and fisheries; Cattle; Biomass; Ecosystem; Phospholipids; Soil Microbiology

Microbial community dynamics associated with manure hot spots were studied by using a model system consisting of gel-stabilized mixture soil and manure, placed between layers soil, during 3-week incubation period. The microbial biomass, measured as the total amount phospholipid fatty acids (PLFA), had doubled within 2-mm distance from soil-manure interface after 3 days. Principal-component analyses demonstrated that this increase was accompanied reproducible changes in composition PLFA, indicating structure. effect strongest 2-mm-thick layer closest to interface, which PLFA statistically significantly different (P < 0.05) unaffected throughout An also observed 2 4 mm interface. biomass structure mainly attributed diffusion dissolved organic carbon manure. During initial period growth, already more abundant than increased core After day 3, these gradually became similar soil. individual suggested both taxonomic physiological occurred growth. Examples latter decreases ratios 16:1 omega 7t 7c cyclopropyl their respective precursors, active bacterial community. inverse relationship eucaryotic 20:4 (arachidonic acid) grazing important.