Evidence of Genomic Instability in Campylobacter jejuni Isolated from Poultry

• DOI: 10.1128/aem.64.5.1816-1821.1998
• Publication Date: 2019-12-19T19:34:55Z
• Authors: Trudy M. Wassenaar; Barbara Geilhausen; Diane G. Newell
• Keywords: Flagellin; DNA profiling

ABSTRACT Poultry isolates of Campylobacter jejuni derived from a survey meat processing batches were genotyped by pulsed-field gel electrophoresis (PFGE) chromosomal DNA to establish the clonal relationships between single-colony isolates. In majority studied, one or two genotype patterns predominated. However, in batch (batch A), 21 gave 14 different PFGE genotypes. The banding obtained with Sma I sufficiently distinguish genotypes, although also produced many common bands. question whether these represented clones had ancestry was addressed additional genotypic and phenotypic methods. Restriction length polymorphism PCR products flagellin genes showed an identical for all contrast, unrelated control resulted Moreover, genotypes A Penner serotypes similar biotypes phage types. It concluded that origin diversity most likely originated genomic rearrangements. shown be stable upon subculturing vitro after vivo passage chickens, natural transformation isogenic mutants carrying antibiotic markers did not occur chick colonization model. possible mechanisms hypothesized recombinations conditions allow, induce, select such events are discussed.