Anaerobic Naphthalene Degradation by a Sulfate-Reducing Enrichment Culture

• DOI: 10.1128/aem.66.7.2743-2747.2000
• Publication Date: 2002-07-27T09:55:30Z
• Authors: Rainer U. Meckens...; Eva Annweiler; Walter Michaelis; Hans H. Richnow; Bernhard Schink
• Keywords: Phenylacetic acid; Benzoic acid; Carboxylation; Phthalic acid; Enrichment culture

ABSTRACT Anaerobic naphthalene degradation by a sulfate-reducing enrichment culture was studied substrate utilization tests and identification of metabolites gas chromatography-mass spectrometry. In tests, the able to oxidize naphthalene, 2-methylnaphthalene, 1- 2-naphthoic acids, phenylacetic acid, benzoic cyclohexanecarboxylic cyclohex-1-ene-carboxylic acid with sulfate as electron acceptor. Neither hydroxylated or derivatives 2-naphthol nor monoaromatic compounds ortho -phthalic 2-carboxy-1-phenylacetic salicylic were utilized within 100 days. 2-Naphthoic accumulated in all naphthalene-grown cultures. Reduced could be identified comparison mass spectra coelution commercial reference such 1,2,3,4-tetrahydro-2-naphthoic chemically synthesized decahydro-2-naphthoic acid. 5,6,7,8-Tetrahydro-2-naphthoic octahydro-2-naphthoic tentatively their spectra. The suggest stepwise reduction aromatic ring system before cleavage. experiments [1- 13 C]naphthalene deuterated D 8 -naphthalene, mentioned derived from introduced labeled naphthalene. When [ C]bicarbonate-buffered growth medium used conjunction unlabeled C incorporation into carboxylic group shown, indicating that activation carboxylation initial step. No fission products identified.