Burkholderia , a Genus Rich in Plant-Associated Nitrogen Fixers with Wide Environmental and Geographic Distribution

• DOI: 10.1128/aem.67.6.2790-2798.2001
• Publication Date: 2002-07-27T09:56:57Z
• Authors: Paulina Estrada-D...; Rocı́o Bustillos-...; Jesús Caballero-...
• Keywords: DNA, Bacterial; 2. Zero hunger; 0301 basic medicine; Geography; Burkholderia; 15. Life on land; Coffee; Plant Roots; Zea mays; Bacterial Typing Techniques; Magnoliopsida; 03 medical and health sciences; Phenotype; Bacterial Proteins; Genes, Bacterial; Nitrogen Fixation; Mexico; Soil Microbiology

ABSTRACT The genus Burkholderia comprises 19 species, including vietnamiensis which is the only known N 2 -fixing species of this bacterial genus. first isolates B. were recovered from rhizosphere rice plants grown in a phytotron, but its existence natural environments and geographic distribution not reported. In present study, most environment field-grown maize coffee cultivated widely separated regions Mexico phenotypically identified as cepacia using API 20NE system. Nevertheless, number these inside roots, well rhizoplane plants, showed similar or identical features to those TVV75 T . These include nitrogenase activity with 10 different carbon sources, very nifHDK hybridization patterns, protein electrophoregrams, amplified 16S rDNA restriction (ARDRA) profiles, levels DNA-DNA reassociation higher than 70% total DNA strain Although ability fix reported be common feature among , results obtained show that many diazotrophic analyzed phenotypic genotypic kururiensis bacterium study species. assays confirmed addition, shows wide substantial capability spp. for colonizing diverse host distantly environments.