Cloning and Characterization of lin Genes Responsible for the Degradation of Hexachlorocyclohexane Isomers by Sphingomonas paucimobilis Strain B90

• DOI: 10.1128/aem.68.12.6021-6028.2002
• Publication Date: 2002-11-26T00:04:48Z
• Authors: Rekha Kumari; Sanjukta Subudhi; Mrutyunjay Suar; Gauri Dhingra; Vishakha Raina; Charu Dogra; Sukanya Lal; Jan Roelof van de...; Christof Holliger; Rup Lal
• Keywords: 0301 basic medicine; 03 medical and health sciences; Bacterial Proteins; Genes, Bacterial; Hydrolases; Escherichia coli; Lyases; Cloning, Molecular; Sphingomonas; Hexachlorocyclohexane; 3. Good health

ABSTRACT

Hexachlorocyclohexane (HCH) has been used extensively against agricultural pests and in public health programs for the control of mosquitoes. Commercial formulations of HCH consist of a mixture of four isomers, α, β, γ, and δ. While all these isomers pose serious environmental problems, β-HCH is more problematic due to its longer persistence in the environment. We have studied the degradation of HCH isomers by Sphingomonas paucimobilis strain B90 and characterized the lin genes encoding enzymes from strain B90 responsible for the degradation of HCH isomers. Two nonidentical copies of the linA gene encoding HCH dehydrochlorinase, which were designated linA1 and linA2 , were found in S. paucimobilis B90 . The linA1 and linA2 genes could be expressed in Escherichia coli , leading to dehydrochlorination of α-, γ-, and δ-HCH but not of β-HCH, suggesting that S. paucimobilis B90 contains another pathway for the initial steps of β-HCH degradation. The cloning and characterization of the halidohydrolase ( linB ), dehydrogenase ( linC and linX ), and reductive dechlorinase ( linD ) genes from S. paucimobilis B90 revealed that they share ∼96 to 99% identical nucleotides with the corresponding genes of S. paucimobilis UT26. No evidence was found for the presence of a linE -like gene, coding for a ring cleavage dioxygenase, in strain B90. The gene structures around the linA1 and linA2 genes of strain B90, compared to those in strain UT26, are suggestive of a recombination between linA1 and linA2 , which formed linA of strain UT26.