Culture-Independent Analysis of Gut Bacteria: the Pig Gastrointestinal Tract Microbiota Revisited

• DOI: 10.1128/aem.68.2.673-690.2002
• Publication Date: 2003-02-26T15:22:46Z
• Authors: Thomas D. Leser; Joanna Z. Amenuvor; Tim K. Jensen; Rikke H. Lindecrona; Mette Boye; Kristian Møller
• Keywords: Phylotype; Prevotella; Phylogenetic diversity

ABSTRACT The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total 24 representing variety diets, ages, and herd health status. A library comprising 4,270 cloned rDNA sequences obtained directly PCR 52 samples either ileum, cecum, or colon constructed. In total, 375 phylotypes identified using 97% similarity criterion. Three hundred nine (83%) had <97% to any database may represent yet-uncharacterized genera species. affiliated with 13 major lineages. four (81%) belonged low-G+C gram-positive division, 42 (11.2%) Bacteroides Prevotella group. Four clusters branching off deeply within bacteria one Mycoplasma without cultured representatives found. coverage all 97.2%. relative abundance clones approximated lognormal distribution; however, detected their varied between two libraries same sample. results document that microbial is very complex majority species colonizing gastrointestinal tract have not been characterized.