Tri13 and Tri7 Determine Deoxynivalenol- and Nivalenol-Producing Chemotypes of Gibberella zeae

• DOI: 10.1128/aem.68.5.2148-2154.2002
• Publication Date: 2002-07-27T10:09:29Z
• Authors: Theresa Lee; You-Kyoung Han; Kook-Hyung Kim; Sung-Hwan Yun; Yin-Won Lee
• Keywords: Chemotype; Gibberella zeae; Gibberella; Vomitoxin

ABSTRACT Gibberella zeae , a major cause of cereal scab, can be divided into two chemotypes based on production the 8-ketotrichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned sequenced Tri13 homolog from each chemotype. The NIV chemotype strain (88-1) is located in trichothecene gene cluster carries an open reading frame similar to that Fusarium sporotrichioides whereas DON (H-11) several mutations. To confirm roles Tri7 genes by G. we genetically altered toxin 88-1 H-11. In transgenic strains, targeted deletion genome caused rather than NIV. Heterologous expression alone or combination with conferred H-11 ability synthesize NIV; latter case, 4-acetylnivalenol (4-ANIV) also was produced. These results suggest are required for oxygenation acetylation oxygen at C-4 during synthesis 4-ANIV . functional analyses provide first clear evidence genetic basis