Nitrous Oxide Reductase ( nosZ ) Gene Fragments Differ between Native and Cultivated Michigan Soils

• DOI: 10.1128/aem.70.1.301-309.2004
• Publication Date: 2004-01-07T21:02:54Z
• Authors: Blaž Stres; Ivan Mahne; Gorazd Avguštin; James M. Tiedje
• Keywords: Nitrous-oxide reductase; Soil microbiology

The effect of standard agricultural management on the genetic heterogeneity nitrous oxide reductase (nosZ) fragments from denitrifying prokaryotes in native and cultivated soil was explored. Thirty-six cores were composited each two conditions. nosZ gene amplified triplicate samples, PCR products cloned screened by restriction fragment length polymorphism (RFLP). total RFLP profiles increased similarity with sample size until 3-g samples produced visually identical for treatment. Large differences observed between soils. representing major groups clones encountered at least twice four randomly selected unique patterns sequenced to verify identity. sequence diversity field higher, only eight found clone libraries both soils among 182 distinct identified A group that comprised 32% all dominated library soil, whereas many minor soil. 95% confidence intervals Chao1 nonparametric richness estimator data did not overlap, indicating levels species are significantly different soils, having higher diversity. Phylogenetic analysis deduced amino acid sequences grouped majority into an interleaved Michigan cluster whose cultured members alpha-Proteobacteria. Only one related gamma-Proteobacteria. Sequences formed a distinct, closely (D(mean) = 0.16) containing 91.6% clones. Clones more distantly other 0.26), 65% outside further difference communities. Overall, there appears be relationship use richness, diversity, phylogenetic position sequences, caused shift diverse community.