In Vitro and In Vivo Inhibition of Helicobacter pylori by Lactobacillus casei Strain Shirota

• DOI: 10.1128/aem.70.1.518-526.2004
• Publication Date: 2004-01-07T21:02:54Z
• Authors: D. Sgouras; P. Maragkoudakis; K. Petraki; B. Martinez-Gonzalez; E. Eriotou; S. Michopoulos; G. Kalantzopoulos; E. Tsakalidou; Α. Mentis
• Keywords: Lactobacillus casei; Strain (injury)

ABSTRACT We studied the potential inhibitory effect of Lactobacillus casei strain Shirota (from fermented milk product Yakult [Yakult Ltd., Tokyo, Japan]) on Helicobacter pylori by using (i) in vitro inhibition assays with H. SS1 (Sydney 1) and nine clinical isolates (ii) vivo mouse model infection over a period 9 months. In activity against all was observed presence viable L. cells but not cell-free culture supernatant, although there profound urease activity. experiments were performed oral administration water supply months to 6-week-old C57BL/6 mice previously infected (study group; n = 25). Appropriate control groups -infected untreated animals ( 25) uninfected given also included study. colonization development gastritis assessed at 1, 2, 3, 6, postinfection. A significant reduction levels antrum body mucosa lactobacillus-treated study group, as cultures, compared group. This accompanied decline associated chronic active gastric mucosal inflammation each time point throughout observation period. trend toward decrease anti- immunoglobulin G response measured serum treated lactobacillus, this significant.