Inactivation of Caliciviruses

• DOI: 10.1128/aem.70.8.4538-4543.2004
• Publication Date: 2004-08-04T17:08:33Z
• Authors: Erwin Duizer; Paul Bijkerk; Barry Rockx; Astrid de Groot; Fleur Twisk; Marion Koopmans
• Keywords: Feline calicivirus; Infectivity; Sodium hypochlorite; Murine norovirus; Caliciviridae

ABSTRACT The viruses most commonly associated with food- and waterborne outbreaks of gastroenteritis are the noroviruses. lack a culture method for noroviruses warrants use cultivable model to gain more insight on their transmission routes inactivation methods. We studied reported enteric canine calicivirus no. 48 (CaCV) respiratory feline F9 (FeCV) correlated reduction in PCR units FeCV, CaCV, norovirus. Inactivation suspended was temperature time dependent range from 0 100°C. UV-B radiation 150 mJ/cm 2 caused dose-dependent inactivation, 3 D ( = 1 log 10 ) infectivity at 34 both viruses. by 70% ethanol inefficient, only after 30 min. Sodium hypochlorite solutions were effective >300 ppm. FeCV showed higher stability pH <3 >7 than CaCV. For all treatments, detection viral RNA underestimated infectivity. Norovirus never sensitive animal caliciviruses profoundly resistant low high pH. Overall, similar profiles when exposed heat or incubated hypochlorite. CaCV suggests that this is not typical (calici-) virus. incomplete concentration needed sufficient virus point concern decontamination fomites surfaces contaminated virus-safe water.