Degradation of 4-Chloro-2-Methylphenoxyacetic Acid in Top- and Subsoil Is Quantitatively Linked to the Class III tfdA Gene

• DOI: 10.1128/aem.72.2.1476-1486.2006
• Publication Date: 2006-02-03T21:18:24Z
• Authors: Jacob Bælum; Trine Henriksen; Hans Christian Br...; Carsten Suhr Jaco...
• Keywords: Subsoil; Topsoil

ABSTRACT The tfdA gene is known to be involved in the first step of degradation phenoxy acid herbicide 4-chloro-2-methylphenoxyacetic (MCPA) several soil bacteria, but bacteria containing other -like genes have been isolated as well. A quantitative real-time PCR method was used monitor increase concentration during MCPA sandy topsoil and subsoil over a period 115 days. Quantitative revealed growth -containing bacterial community, from 500 g −1 approximately 3 × 10 4 7 5 for initially added 2.3 mg kg (dry weight) 20 soil, respectively. We analyzed diversity experiment. Analyses melting curves amplification products showed that shift dominant population structure occurred period. Further denaturing gradient gel electrophoresis sequence analysis responsible belonged class III genes, while present before occurrence I genes. implications these results initial assessment functional soils does not necessarily reflect organisms or would carry out compounds question.