Global Carbon Utilization Profiles of Wild-Type, Mutant, and Transformant Strains of Hypocrea jecorina

• DOI: 10.1128/aem.72.3.2126-2133.2006
• Publication Date: 2006-03-03T17:47:56Z
• Authors: Irina S. Druzhinina; Monika Schmoll; Bernhard Seiboth; Christian P. Kubicek
• Keywords: Hypocrea; Wild type

ABSTRACT The ascomycete Hypocrea jecorina ( Trichoderma reesei ), an industrial producer of cellulases and hemicellulases, can efficiently degrade plant polysaccharides. However, the catabolic pathways for resulting monomers their relationship to enzyme induction are not well known. Here we used Biolog Phenotype MicroArrays technique evaluate growth H. on 95 carbon sources. For this purpose, compared several wild-type isolates, mutants producing different amounts cellulases, strains transformed with a heterologous antibiotic resistance marker gene. isolates had highest variation in patterns individual cellulase were relatively similar parental strains. Both mutant strains, most significant changes occurred utilization xylitol, erythritol, d -sorbitol, -ribose, -galactose, l -arabinose, N -acetyl- -glucosamine, maltotriose, β-methyl-glucoside. Increased production was negatively correlated ability grow γ-aminobutyrate, adonitol, 2-ketogluconate; positively that -sorbitol saccharic acid. reproducibility, relative simplicity, high resolution (±10% increase mycelial density) phenotypic microarrays make them useful tool characterization global analysis gene function.