Development and Validation of an Oligonucleotide Microarray for Detection of Multiple Virulence and Antimicrobial Resistance Genes in Escherichia coli

• DOI: 10.1128/aem.72.5.3780-3784.2006
• Publication Date: 2006-05-04T02:49:21Z
• Authors: Guillaume Bruant; Christine Maynard; Sadjia Bekal; Isabelle Gaucher; Luke Masson; Roland Brousseau; Josée Harel
• Keywords: 0301 basic medicine; Virulence; Escherichia coli Proteins; Gene Expression Profiling; environmental; 3. Good health; virulence; 03 medical and health sciences; Drug Resistance, Bacterial; Escherichia coli; Animals; Humans; genes; Oligonucleotide Array Sequence Analysis

ABSTRACT An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance was designed validated using DNA from known reference strains. This confirmed to be a powerful diagnostic tool for monitoring emerging E. pathotypes resistance, as well environmental, epidemiological, phylogenetic studies including the evaluation of genome plasticity.