Lactobacillus johnsoniiLa1 AttenuatesHelicobacter pylori-Associated Gastritis and Reduces Levels of Proinflammatory Chemokines in C57BL/6 Mice

• DOI: 10.1128/cdli.12.12.1378-1386.2005
• Publication Date: 2005-12-07T20:07:41Z
• Authors: Dionyssios N. Sgo...; Effrosini G. Pana...; Beatriz Martinez-...; Kalliopi Petraki; Spyros Michopoulos; Αndreas Mentis
• Keywords: Proinflammatory cytokine; Lamina propria; Helicobacter

In clinical settings, Lactobacillus johnsonii La1 administration has been reported to have a favorable effect on Helicobacter pylori-associated gastritis, although the mechanism remains unclear. We administered, continuously through water supply, live H. pylori-infected C57BL/6 mice and followed colonization, development of gastritis in lamina propria, levels proinflammatory chemokines macrophage inflammatory protein 2 (MIP-2) keratinocyte-derived cytokine (KC) serum gastric tissue over period 3 months. documented significant attenuation both lymphocytic (P=0.038) neutrophilic (P=0.003) infiltration propria as well circulating anti-H. pylori immunoglobulin G antibodies (P=0.003), we did not observe suppressive colonizing numbers. Other lactobacilli, such L. amylovorus DCE 471 acidophilus IBB 801, attenuate same extent. MIP-2 were distinctly reduced during early stages infection La1-treated animals, mucosal KC. Finally, also observed reduction (P=0.046) pylori-induced interleukin-8 secretion by human adenocarcinoma AGS cells vitro presence neutralized (pH 6.8) spent culture supernatants, without concomitant loss viability. These observations suggest that stages, can vivo, possibly reducing chemotactic signals responsible for recruitment lymphocytes neutrophils propria.