Ceramidase Activity in Bacterial Skin Flora as a Possible Cause of Ceramide Deficiency in Atopic Dermatitis

• DOI: 10.1128/cdli.6.1.101-104.1999
• Publication Date: 2019-12-31T19:22:01Z
• Authors: Yoshinori Ohnishi; Nozomu Okino; Makoto Ito; Shuhei Imayama
• Keywords: Sphingolipid; Skin flora; Epidermis (zoology); Phosphorylcholine

ABSTRACT A marked decrease in the content of ceramide has been reported horny layer epidermis atopic dermatitis (AD). This impairs permeability barrier epidermis, resulting characteristic dry and easily antigen-permeable skin AD, since serves as major water-holding molecule extracellular space layer. On other hand, such patients is frequently colonized by bacteria, most typically Staphylococcus aureus , possessing genes those for sphingomyelinase, which are related to sphingolipid metabolism. We therefore tried identify a possible correlation between bacterial flora obtained from 25 with AD versus that 24 healthy subjects, using thin-layer chromatographic assay sphingomyelin-associated enzyme activities secreted bacteria. The findings demonstrated ceramidase, breaks down into sphingosine fatty acid, was significantly more both lesional nonlesional than subjects; sphingomyelin phosphorylcholine, all types at similar levels controls. finding ceramidase-secreting bacteria thus suggests microorganisms deficiency increases hypersensitivity impairing barrier.