Calcein-Acetyoxymethyl Cytotoxicity Assay: Standardization of a Method Allowing Additional Analyses on Recovered Effector Cells and Supernatants

• DOI: 10.1128/cdli.8.6.1131-1135.2001
• Publication Date: 2002-07-27T06:03:53Z
• Authors: Simona Neri; Erminia Mariani; Alessandra Menegh...; Luca Cattini; Andrea Facchini
• Keywords: Calcein; Lytic cycle

ABSTRACT Cytotoxicity assays provide an in vitro evaluation of the lytic activity NK and T cells against tumors or transformed cells. However, none these methods allow recovery supernatants after assay. We standardized a microcytotoxicity test using calcein-acetoxymethyl (calcein-AM) dye that requires very small quantities while maintaining same sensitivity as traditional 51 Cr The assay is applicable to resting well activated human effector uses different targets such cell lines are adherent growing suspension resistant sensitive. most important feature method possibility recovering for additional analyses phenotyping soluble factors.