Antibodies to 60-Kilodalton Heat Shock Protein and Outer Membrane Protein 2 of Chlamydia pneumoniae in Patients with Coronary Heart Disease

• DOI: 10.1128/cdli.9.1.66-74.2002
• Publication Date: 2002-07-27T09:55:30Z
• Authors: Alessandra Ciervo; Paolo Visca; Andrea Petrucca; Luigi Maria Biasucci; Attilio Maseri; Antonio Cassone
• Keywords: Chlamydophila pneumoniae; HSP60; Antibody titer

ABSTRACT Evidence linking Chlamydia pneumoniae infection to atherosclerosis and atherothrombotic events has recently emerged. A primary candidate implicated in these pathogenetic is the 60-kDa chlamydial heat shock protein (HSP60). Another putative activate a potential proinflammatory mechanism outer membrane 2 (OMP2). We have generated both HSP60 OMP2 recombinant antigens nondenatured form shown that (i) two were highly immunogenic mice (ii) murine antisera thus recognized native C. proteins. measured by enzyme linked immunosorbent assay (ELISA) immunoblot antibody titers samples from 219 patients with coronary heart disease (CHD), 179 unstable angina (UA), 40 acute myocardial infarction (AMI), 100 age-, sex-, risk factor-matched healthy controls. also examined whether anti-HSP60 and/or anti-OMP2 antibodies correlated anti- assessed commercial microimmunofluorescence (MIF) assay. Immunoglobulin G (IgG), but neither IgA nor IgM, against proteins detected ELISA. In particular, >99% of CHD versus 0% controls, whereas proportions positive subjects >70 27%, respectively. Nonetheless, among patients, similar frequencies or present UA AMI subjects. The anti-OMP2, not anti-HSP60, showed high specificity. Consistently, serological correlation was observed between IgG MIF ELISA reactivity HSP60. Overall, results this study demonstrate strong levels, as our in-house They suggest ELISA, because its specificity assay, could be diagnostic marker for infection, which would usefulness nonsubjective nature.