Gene Expression of Nucleic Acid-Sensing Pattern Recognition Receptors in Children Hospitalized for Respiratory Syncytial Virus-Associated Acute Bronchiolitis

• DOI: 10.1128/cvi.00445-08
• Publication Date: 2009-04-23T00:55:42Z
• Authors: Carolina Scagnolari; Fabio Midulla; Alessandra Pieran...; Corrado Moretti; Enea Bonci; Rosaria Berardi; Daniela De Angelis; Carla Selvaggi; Paola Di Marco; Enrico Girardi; Guido Antonelli
• Keywords: Rhinovirus; TLR3; RIG-I

ABSTRACT Given the critical role of pattern recognition receptors (PRRs) in acid nucleic initiation innate immunity and orchestration adaptive immunity, aim this study was to determine whether any heterogeneity PRR expression airway tracts infants with respiratory syncytial virus (RSV) infection might explain broad clinical spectrum RSV-associated bronchiolitis infants. For purpose, levels melanoma differentiation-associated protein-5 (MDA-5), retinoic inducible gene-1 (RIG-1), Toll-like receptor 3 (TLR-3), TLR-7, TLR-8, TLR-9 mRNAs were evaluated, using TaqMan quantitative reverse transcription-PCR, cells from nasopharyngeal washes collected 157 suffering acute or not they associated viruses. High interindividual variability observed both virus-positive -negative infants; however, relative gene MDA-5, RIG-1, TLR-8 significantly higher virus-infected group, whereas TLR-3 different. The differences more evident RSV than those bocavirus rhinovirus infection. In RSV-infected infants, PRR-mRNA also analyzed relation interferon protein levels, viral load, severity, days hospitalization, age, body weight. A significant positive correlation only between load RIG-1 mRNA levels. These findings provide first direct evidence that, virus-associated bronchiolitis, especially RSV, there are substantial changes expression; likely is an important determinant outcome bronchiolitis.