Dynamic Changes in Inflammatory Cytokines in Pigs Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

• DOI: 10.1128/cvi.00517-09
• Publication Date: 2010-07-15T03:46:59Z
• Authors: Yonggang Liu; Wenda Shi; Enmin Zhou; Shujie Wang; Shouping Hu; Xuehui Cai; Fulong Rong; Jiabin Wu; Min Xu; Mingming Xu; Liqin Li
• Keywords: Inflammation; 0301 basic medicine; Swine; Body Weight; Respiratory System; Porcine Reproductive and Respiratory Syndrome; Appetite; Viral Load; Body Temperature; Cell Line; 3. Good health; 03 medical and health sciences; Animals; Cytokines; Porcine respiratory and reproductive syndrome virus; Viremia; Serial Passage; Lung Diseases, Interstitial

Porcine reproductive and respiratory syndrome virus (PRRSV) infection induces both humoral cellular immune responses. In this study, we investigated the changes in cytokine levels peripheral blood between highly pathogenic PRRSV HuN4 strain its derivative HuN4-F112 obtained by serial propagation MARC145 cells to 112 passages. The results demonstrated that pigs infected with showed a loss of appetite, decrease body weight, raised temperature, symptoms, along interstitial pneumonia lesions. amounts were 10(5) 10(9) copies/ml 10(10) 10(11) copies/g lung tissues, whereas 10(2.15) 10(3.13) 10(3.0) 10(3.6) lungs. Moreover, interleukin 1 (IL-1), IL-6, tumor necrosis factor alpha (TNF-alpha), interferon (IFN-alpha) upregulated 7 days postinoculation HuN4, which was earlier than group. Furthermore, returned normal on 21st day postinoculation, while those continued increase. These generated higher inflammatory cytokines, also indicated may aggravate inflammation damage tissues organs. low-pathogenic induced lower enhance responses against infection.