The Hog1 Mitogen-Activated Protein Kinase Is Essential in the Oxidative Stress Response and Chlamydospore Formation inCandidaalbicans

• DOI: 10.1128/ec.2.2.351-361.2003
• Publication Date: 2003-04-08T22:34:33Z
• Authors: Rebeca Alonso-Monge; Federico Navarro-...; Elvira Román; Ana I. Negredo; Blanca Eisman; César Nombela; Jesús Pla
• Keywords: Cell Nucleus; Organelles; 0303 health sciences; Saccharomyces cerevisiae Proteins; Cell Death; MAP Kinase Signaling System; Ultraviolet Rays; Osmolar Concentration; Cell Cycle Proteins; Spores, Fungal; Oxidants; Fungal Proteins; Oxidative Stress; 03 medical and health sciences; Basic-Leucine Zipper Transcription Factors; Candida albicans; Mutation; Mitogen-Activated Protein Kinases; Phosphorylation; Reactive Oxygen Species; Cells, Cultured

ABSTRACT Candida albicans mutants with mutations in mitogen-activated protein (MAP) kinase HOG1 displayed an increased sensitivity to agents producing reactive oxygen species, such as oxidants (menadione, hydrogen peroxide, or potassium superoxide), and UV light. Consistent this finding, C . Hog1 was activated not only response increase external osmolarity, happens its Saccharomyces cerevisiae homologue, but also peroxide. The Hog1-mediated oxidative stress different from that of transcription factor Cap1, the homologue S Yap1, shown by sensitivities kinetics cell death cap1 Δ, hog1 , Δ mutants. Deletion CAP1 did influence level phosphorylation, deletion affect Cap1 nuclear localization. Moreover, we show gene plays a role chlamydospore formation, another oxygen-related morphogenetic event, demonstrated fact cells were unable generate these thick-walled structures several media through mechanism EFG1 regulator. This is first demonstration MAP pathway resistance pathogenic fungi, it allows us propose molecular model for