Candida albicans VPS11 Is Required for Vacuole Biogenesis and Germ Tube Formation

• DOI: 10.1128/ec.2.3.411-421.2003
• Publication Date: 2003-06-09T23:12:20Z
• Authors: Glen E. Palmer; Annette Cashmore; Joy Sturtevant
• Keywords: Germ tube

The Candida albicans vacuole has previously been observed to undergo rapid expansion during the emergence of a germ tube from yeast cell, occupy majority parent cell. Furthermore, yeast-to-hypha switch implicated in virulence this organism. class C vps (vacuolar protein sorting) mutants Saccharomyces cerevisiae are defective multiple delivery pathways and prevacuole compartment. In study C. homologues S. VPS genes have identified. Deletion VPS11 homologue resulted number phenotypes that closely resemble those cerevisiae, including absence vacuolar vps11Delta mutant also had much-reduced secreted lipase aspartyl protease activities. strains were yeast-hypha morphogenesis. Upon serum induction filamentous growth, showed delayed tubes, reduced apical extension rate compared control strains, unable form mature hyphae. These results suggest Vps11p-mediated trafficking steps necessary support