Comparative Analysis of EspF Variants in Inhibition of Escherichia coli Phagocytosis by Macrophages and Inhibition of E. coli Translocation through Human- and Bovine-Derived M Cells

• DOI: 10.1128/iai.00023-11
• Publication Date: 2011-08-30T00:56:23Z
• Authors: Amin Tahoun; Gabriella Siszler; Kevin Spears; Sean McAteer; Jai Tree; Edith Paxton; Trudi L. Gillespie; Isabel Martinez-A...; Mark A. Jepson; Darren J. Shaw; Manfred Koegl; Juergen Haas; David L. Gally; Arvind Mahajan
• Keywords: Enteropathogenic Escherichia coli

ABSTRACT The EspF protein is secreted by the type III secretion system of enteropathogenic and enterohemorrhagic Escherichia coli (EPEC EHEC, respectively). sequences differ between EHEC O157:H7, O26:H11, EPEC O127:H6 in terms number SH3-binding polyproline-rich repeats specific residues these regions, as well amino domain involved cellular localization. O127 important for inhibition phagocytosis also limits translocation through antigen-sampling cells (M cells). has been shown to have effects on organelle function interacts with several host proteins, including N-WASP sorting nexin 9 (SNX9). In this study, we compared capacities different espF alleles inhibit (i) bacterial macrophages, (ii) an M-cell coculture system, (iii) uptake cultured bovine epithelial cells. gene from E. serotype O157 ( ) allele was significantly less effective at inhibiting had reduced capacity a human-derived vitro comparison O26 . contrast, most restricting into primary terminal rectum, predominant colonization site cattle containing M-like Although LUMIER binding assays demonstrated differences interactions variants SNX9 N-WASP, propose that other, as-yet-uncharacterized contribute host-based variation activity here.