Staphylococcus aureus CcpA Affects Biofilm Formation

• DOI: 10.1128/iai.00035-08
• Publication Date: 2008-03-18T00:48:32Z
• Authors: Kati Seidl; Christiane Goerke; Christiane Wolz; Dietrich Mack; Brigitte Berger-B...; Markus Bischoff
• Keywords: CCPA

ABSTRACT Biofilm formation in Staphylococcus aureus under vitro growth conditions is generally promoted by high concentrations of sugar and/or salts. The addition glucose to routinely used complex media triggered biofilm S. strain SA113. Deletion ccpA , coding for the catabolite control protein A (CcpA), which regulates gene expression response carbon source, abolished capacity SA113 form a static and flow conditions, while still allowing primary attachment polystyrene surfaces. This suggested that CcpA mainly affects accumulation intercellular aggregation. trans -Complementation mutant with wild-type allele fully restored formation. produced was susceptible sodium metaperiodate, DNase I, proteinase K treatment, indicating presence polysaccharide adhesin (PIA), factors, extracellular DNA (eDNA). investigation several factors were reported influence ( arlRS mgrA rbf sarA atl ica citZ citB cidABC ) showed up-regulated transcription cidA recently shown contribute eDNA production. Moreover, we increased icaA PIA production, presumably over down-regulation tricarboxylic acid cycle genes .