Identification of Markers forHelicobacter pyloriStrains Isolated from Children with Peptic Ulcer Disease by Suppressive Subtractive Hybridization

• DOI: 10.1128/iai.00123-06
• Publication Date: 2006-06-21T23:10:29Z
• Authors: Mónica Oleastro; Lurdes Monteiro; Philippe Lehours; Francis Mégraud; Armelle Ménard
• Keywords: Suppression subtractive hybridization; Spirillaceae; Helicobacter; CagA

Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the can develop earlier, and rare cases have been observed in children, suggesting that these H. strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between isolated from 5-year-old child with duodenal sex- age-matched gastritis only. The prevalence of 30 tester-specific subtracted sequences was determined on collection children (15 ulcers gastritis) adults (46 44 gastritis). Two sequences, jhp0562 (80.0% versus 33.3%, P = 0.008) jhp0870 36.7%, 0.015), were highly associated PUD third sequence, jhp0828, less (40.0% 10.0%, 0.048). Among adult strains, none PUD. both prevalent adenocarcinoma than adults, difference being statistically significant jhp0870. In conclusion, two genes identified as strongly their putative roles an outer membrane protein lipopolysaccharide biosynthesis jhp0562, suggest they novel virulence factors pylori.