Distinct Intensity of Host-Pathogen Interactions in Chlamydia psittaci- and Chlamydia abortus-Infected Chicken Embryos

• DOI: 10.1128/iai.00437-12
• Publication Date: 2012-06-12T05:40:29Z
• Authors: Maria Braukmann; Konrad Sachse; Ilse D. Jacobsen; Martin Westermann; Christian Menge; Hans-Peter Saluz; Angela Berndt
• Keywords: Chlamydia psittaci; Chlamydophila; Embryonated; Host adaptation

ABSTRACT Factors and mechanisms determining the differences in virulence host specificity between zoonotic agents Chlamydia psittaci abortus are still largely unknown. In present study, two strains were compared for their invasiveness, virulence, capability of eliciting an immune response chicken embryos. On breeding day 10, embryonated eggs inoculated with 5 × 10 4 inclusion-forming units. As shown by immunohistochemistry quantitative real-time PCR, C. displayed a significantly better disseminating chorioallantoic membrane (CAM) internal organs than . The higher infectious potential birds was underlined mRNA expression rates essential chlamydial genes, such as incA , groEL (in CAM, liver, spleen), cpaf ftsW CAM). Although responses to both pathogens similar, elicited macrophage numbers stronger subset immune-related proteins. data imply that invasiveness spp. propagation not solely dependent on level but, even greater extent, bacterial factors related virulence. fact has coped far avian embryo's upregulating genes may be key understanding underlying adaptation etiopathology.