Tim-3 Induces Th2-Biased Immunity and Alternative Macrophage Activation during Schistosoma japonicum Infection

• DOI: 10.1128/iai.00517-15
• Publication Date: 2015-05-19T02:11:13Z
• Authors: Nan Hou; Xianyu Piao; Shuai Liu; Chuang Wu; Qijun Chen
• Keywords: Male; 0301 basic medicine; Macrophages; Immunity; CD8-Positive T-Lymphocytes; Macrophage Activation; Schistosoma japonicum; 3. Good health; Killer Cells, Natural; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Th2 Cells; Liver; Schistosomiasis japonica; Animals; Humans; Receptors, Virus; Hepatitis A Virus Cellular Receptor 2; Spleen

T cell immunoglobulin- and mucin-domain-containing molecule 3 (Tim-3) has been regarded as an important regulatory factor in both adaptive innate immunity. Recently, Tim-3 was reported to be involved Th2-biased immune responses mice infected with Schistosoma japonicum, but the exact mechanism behind involvement of remains unknown. The present study aims understand role response against S. japonicum infection. expression determined by flow cytometry, increased observed on CD4(+) CD8(+) cells, NK1.1(+) CD11b(+) cells from livers japonicum-infected mice. However, level lower spleen than liver, no increase splenic or cells. schistosome-induced upregulation natural killer (NK) accompanied reduced NK numbers vitro vivo. antibody blockade led inducible nitric oxide synthase interleukin-12 (IL-12) mRNA cocultured soluble egg antigen downregulation Arg1 IL-10, which are markers M2 macrophages. In summary, we critical populations, may alternative activation macrophages during