Role of Nod1 in Mucosal Dendritic Cells during Salmonella Pathogenicity Island 1-Independent Salmonella enterica Serovar Typhimurium Infection

• DOI: 10.1128/iai.00519-09
• Publication Date: 2009-07-21T00:58:43Z
• Authors: Lionel Le Bourhis; Joao Gamelas Maga...; Thirumahal Selvan...; Leonardo H. Trava...; Kaoru Geddes; Jörg H. Fritz; Jérôme Viala; Karsten Tedin; Stephen E. Girardin; Dana J. Philpott
• Keywords: NOD1; Lamina propria; Salmonella enterica; Intracellular parasite; CD11c

ABSTRACT Recent advances in immunology have highlighted the critical function of pattern-recognition molecules (PRMs) generating innate immune response to effectively target pathogens. Nod1 and Nod2 are intracellular PRMs that detect peptidoglycan motifs from cell walls bacteria once they gain access cytosol. Salmonella enterica serovar Typhimurium is an enteric pathogen causes a severe disease mouse model. This resides within vacuoles inside cell, but question whether cytosolic such as could impact on course S . infection vivo has not been addressed. Here, we show deficiency PRM Nod1, Nod2, resulted increased susceptibility toward mutant strain targets directly lamina propria dendritic cells (DCs) for its entry into host. Using this bacterium bone marrow chimeras, uncovered surprising role myeloid controlling bacterial at level intestinal propria. Indeed, DCs deficient exhibited impaired clearance bacteria, both vitro vivo, leading organ colonization decreased host survival after oral infection. Taken together, these findings demonstrate key through modulation DCs.