The Staphylococcus aureus Cell Wall-Anchored Protein Clumping Factor A Is an Important T Cell Antigen

• DOI: 10.1128/iai.00549-17
• Publication Date: 2017-09-26T00:30:33Z
• Authors: Keenan A. Lacey; John M. Leech; Stephen J. Lalor; Niamh McCormack; Joan A. Geoghegan; Rachel M. McLoughlin
• Keywords: Coagulase; 0301 basic medicine; 570; Antigens, Bacterial; Staphylococcus aureus; 0303 health sciences; T-Lymphocytes; Staphylococcal Vaccines; Staphylococcal Infections; Survival Analysis; 3. Good health; Mice, Inbred C57BL; Disease Models, Animal; 03 medical and health sciences; 616; Microbial Immunity and Vaccines; Animals; Humans; Cells, Cultured

ABSTRACT Staphylococcus aureus has become increasingly resistant to antibiotics, and vaccines offer a potential solution this epidemic of antimicrobial resistance. Targeting specific T cell subsets is now considered crucial for next-generation anti- S. vaccines; however, there paucity information regarding antigens . This study highlights the importance wall-anchored proteins as human CD4 + activators capable driving antigen-specific Th1 Th17 activation. Clumping factor A (ClfA), which contains N1, N2, N3 binding domains, was found be potent activator. We further investigated subdomains ClfA were involved in activation that full-length N123 N23 activators. Interestingly, N1 subdomain exclusively activating cells. Furthermore, when these used model vaccine, offered Th1- Th17-mediated systemic protection mice upon intraperitoneal challenge. Overall, required maximal both locally systemically.